real-time cell analyzer (rtca) software pro 2.3.2  (Agilent technologies)

Journal: Pharmaceuticals

Article Title: Preclinical Efficacy and Toxicity Analysis of the Pan-Histone Deacetylase Inhibitor Gossypol for the Therapy of Colorectal Cancer or Hepatocellular Carcinoma

doi: 10.3390/ph15040438

Figure Lengend Snippet: Reduced proliferation and viability of different hepatoma and colon carcinoma cell lines treated with gossypol. ( a ) Real-time proliferation curves. After 10 h incubation, cells were treated with different concentrations of gossypol (5 µM, 10 µM, 20 µM, 50 µM) or culture medium (negative control) and monitored for an additional 96 h. As control for complete inhibition of cell proliferation, 0.1% ( v / v ) Triton X-100 (positive control) was used. Cellular impedance was measured over the entire observation time using the xCELLigence TM SP device (Agilent Technologies Inc., Santa Clara, CA, USA) and calculated by the RTCA Software Pro (2.3.2.). All CI values were normalized at 10 h before treatment started. Normalized CI values are displayed in intervals of 5 h. The mean values ± SD of three independent experiments are depicted. IC 50 values for treatment duration of 72 h and 96 h were calculated with RTCA Software; HepG2, IC 50 (72 h): 6.30 µM (SD = 1.73 µM), IC 50 (96 h): 4.35 µM (SD = 2.10 µM); Hep3B, IC 50 (72 h): 6.87 µM (SD = 1.02 µM), IC 50 (96 h): 5.83 µM (SD = 0.81 µM); HCT-116, IC 50 (72 h): 3.61 µM (SD = 1.54 µM), IC 50 (96 h): 5.36 µM (SD = 2.87 µM), HT-29, IC 50 (72 h): 11.8 µM (SD = 10.01 µM), IC 50 (96 h): 14.0 µM (SD = 12.01 µM). Investigation of cell viability via SRB assay. Cells were treated for 48 h ( b ) and 96 h ( c ) with increasing concentrations of gossypol (5 µM, 10 µM, 20 µM, 50 µM), with culture medium (negative control) and 0.1% ( v / v ) Triton X-100 (positive control). Values represent means ± SD of three independent experiments, each performed in triplicate. CI, cell index; SRB, sulforhodamine B.

Article Snippet: Cell index values were calculated using the RTCA Software Pro (2.3.2.) (Agilent Technologies Inc.).

Techniques: Incubation, Negative Control, Inhibition, Positive Control, Software, Sulforhodamine B Assay

Journal: Molecules

Article Title: Comparative Analysis of the Antitumor Activity of Cis- and Trans-Resveratrol in Human Cancer Cells with Different p53 Status

doi: 10.3390/molecules26185586

Figure Lengend Snippet: Real time proliferation assay. ( a ) HepG2 and ( b ) Hep3B cells were exposed to different concentrations of trans- and cis-resveratrol (1 µM, 10 µM, 100 µM) or solvent as control and monitored for 96 h by measuring cellular impedance at 30 min intervals. In the graph, 4 h intervals are shown. The xCELLigence ® SP system was used for this purpose. Exposure to 10 µM SAHA was used as positive control and exposure to 1% ( v / v ) Triton X-100 resulted in complete cell death. Data were calculated using RTCA Software Pro 2.3.2 (Agilent Technologies Inc., Santa Clara, CA, USA). For each cell line, the mean values of one representative run out of three, measured as quadruplicates, are shown. Res, resveratrol; SAHA, suberoylanilide hydroxamic acid.

Article Snippet: For data analysis, cell index values were calculated using the Real Time Cell Analyzer (RTCA) Software Pro (2.3.2.) (Agilent Technologies Inc.).

Techniques: Proliferation Assay, Positive Control, Software